1F2L
CRYSTAL STRUCTURE OF CHEMOKINE DOMAIN OF FRACTALKINE
Summary for 1F2L
| Entry DOI | 10.2210/pdb1f2l/pdb |
| Descriptor | FRACTALKINE (2 entities in total) |
| Functional Keywords | chemoattractant, fractalkine, neurotactin, cytokine |
| Biological source | Homo sapiens (human) |
| Cellular location | Cell membrane; Single-pass type I membrane protein. Processed fractalkine: Secreted: P78423 |
| Total number of polymer chains | 4 |
| Total formula weight | 34624.07 |
| Authors | Hoover, D.M.,Mizoue, L.S.,Handel, T.M.,Lubkowski, J. (deposition date: 2000-05-26, release date: 2000-09-06, Last modification date: 2024-10-30) |
| Primary citation | Hoover, D.M.,Mizoue, L.S.,Handel, T.M.,Lubkowski, J. The crystal structure of the chemokine domain of fractalkine shows a novel quaternary arrangement. J.Biol.Chem., 275:23187-23193, 2000 Cited by PubMed Abstract: Fractalkine, or neurotactin, is a chemokine that is present in endothelial cells from several tissues, including brain, liver, and kidney. It is the only member of the CX(3)C class of chemokines. Fractalkine contains a chemokine domain (CDF) attached to a membrane-spanning domain via a mucin-like stalk. However, fractalkine can also be proteolytically cleaved from its membrane-spanning domain to release a freely diffusible form. Fractalkine attracts and immobilizes leukocytes by binding to its receptor, CX(3)CR1. The x-ray crystal structure of CDF has been solved and refined to 2.0 A resolution. The CDF monomers form a dimer through an intermolecular beta-sheet. This interaction is somewhat similar to that seen in other dimeric CC chemokine crystal structures. However, the displacement of the first disulfide in CDF causes the dimer to assume a more compact quaternary structure relative to CC chemokines, which is unique to CX(3)C chemokines. Although fractalkine can bind to heparin in vitro, as shown by comparison of electrostatic surface plots with other chemokines and by heparin chromatography, the role of this property in vivo is not well understood. PubMed: 10770945DOI: 10.1074/jbc.M002584200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
Download full validation report
