1F2L

CRYSTAL STRUCTURE OF CHEMOKINE DOMAIN OF FRACTALKINE

1F2L の概要

• エントリーDOI: 10.2210/pdb1f2l/pdb
• 分子名称: FRACTALKINE (2 entities in total)
• 機能のキーワード: chemoattractant, fractalkine, neurotactin, cytokine
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cell membrane; Single-pass type I membrane protein. Processed fractalkine: Secreted: P78423
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 34624.07

構造登録者

Hoover, D.M.,Mizoue, L.S.,Handel, T.M.,Lubkowski, J. (登録日: 2000-05-26, 公開日: 2000-09-06, 最終更新日: 2024-10-30)

主引用文献

Hoover, D.M.,Mizoue, L.S.,Handel, T.M.,Lubkowski, J.
The crystal structure of the chemokine domain of fractalkine shows a novel quaternary arrangement.
J.Biol.Chem., 275:23187-23193, 2000

PubMed Abstract: Fractalkine, or neurotactin, is a chemokine that is present in endothelial cells from several tissues, including brain, liver, and kidney. It is the only member of the CX(3)C class of chemokines. Fractalkine contains a chemokine domain (CDF) attached to a membrane-spanning domain via a mucin-like stalk. However, fractalkine can also be proteolytically cleaved from its membrane-spanning domain to release a freely diffusible form. Fractalkine attracts and immobilizes leukocytes by binding to its receptor, CX(3)CR1. The x-ray crystal structure of CDF has been solved and refined to 2.0 A resolution. The CDF monomers form a dimer through an intermolecular beta-sheet. This interaction is somewhat similar to that seen in other dimeric CC chemokine crystal structures. However, the displacement of the first disulfide in CDF causes the dimer to assume a more compact quaternary structure relative to CC chemokines, which is unique to CX(3)C chemokines. Although fractalkine can bind to heparin in vitro, as shown by comparison of electrostatic surface plots with other chemokines and by heparin chromatography, the role of this property in vivo is not well understood.

PubMed: 10770945
DOI: 10.1074/jbc.M002584200

実験手法

X-RAY DIFFRACTION (2 Å)