1EZN

SOLUTION STRUCTURE OF A DNA THREE-WAY JUNCTION

1EZN の概要

• エントリーDOI: 10.2210/pdb1ezn/pdb
• 分子名称: DNA THREE-WAY JUNCTION (1 entity in total)
• 機能のキーワード: dna, three-way junction, four-way junction, hmg-box protein, holliday junction, hammerhead
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11053.05

構造登録者

van Buuren, B.N.M.,Overmars, F.J.,Ippel, J.H.,Altona, C.,Wijmenga, S.S. (登録日: 2000-05-11, 公開日: 2001-04-21, 最終更新日: 2024-05-22)

主引用文献

van Buuren, B.N.,Overmars, F.J.,Ippel, J.H.,Altona, C.,Wijmenga, S.S.
Solution structure of a DNA three-way junction containing two unpaired thymidine bases. Identification of sequence features that decide conformer selection.
J.Mol.Biol., 304:371-383, 2000

PubMed Abstract: The solution structure of a DNA three-way junction (3H) containing two unpaired thymidine bases at the branch site (3HT2), was determined by NMR. Arms A and B of the 3HT2 form a quasi-continuous stacked helix, which is underwound at the junction and has an increased helical rise. The unstacked arm C forms an acute angle of approximately 55 degrees with the unique arm A. The stacking of the unpaired thymidine bases on arm C resembles the folding of hairpin loops. From this data, combined with the reported stacking behavior of 23 other 3HS2 s, two rules are derived that together correctly reproduce their stacking preference. These rules predict, from the sequence of any 3HS2, its stacking preference. The structure also suggests a plausible mechanism for structure-specific recognition of branched nucleic acids by proteins.

PubMed: 11090280
DOI: 10.1006/jmbi.2000.4224

実験手法

SOLUTION NMR