1EY1

SOLUTION STRUCTURE OF ESCHERICHIA COLI NUSB

1EY1 の概要

• エントリーDOI: 10.2210/pdb1ey1/pdb
• 分子名称: ANTITERMINATION FACTOR NUSB (1 entity in total)
• 機能のキーワード: transcirption termination, transcription
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15710.03

構造登録者

Altieri, A.S.,Mazzulla, M.J.,Horita, D.A.,Coats, R.H.,Wingfield, P.T.,Byrd, R.A. (登録日: 2000-05-05, 公開日: 2000-06-14, 最終更新日: 2024-05-22)

主引用文献

Altieri, A.S.,Mazzulla, M.J.,Horita, D.A.,Coats, R.H.,Wingfield, P.T.,Das, A.,Court, D.L.,Byrd, R.A.
The structure of the transcriptional antiterminator NusB from Escherichia coli.
Nat.Struct.Biol., 7:470-474, 2000

PubMed Abstract: We have determined the solution structure of NusB, a transcription antitermination protein from Escherichia coli. The structure reveals a novel, all alpha-helical protein fold. NusB mutations that cause a loss of function (NusB5) or alter specificity for RNA targets (NusB101) are localized to surface residues and likely affect RNA-protein or protein-protein interactions. Residues that are highly conserved among homologs stabilize the protein core. The solution structure of E. coli NusB presented here resembles that of Mycobacterium tuberculosis NusB determined by X-ray diffraction, but differs substantially from a solution structure of E. coli NusB reported earlier.

PubMed: 10881193
DOI: 10.1038/75869

実験手法

SOLUTION NMR