1EXZ

STRUCTURE OF STEM CELL FACTOR

1EXZ の概要

• エントリーDOI: 10.2210/pdb1exz/pdb
• 分子名称: STEM CELL FACTOR, CALCIUM ION, SAMARIUM (III) ION, ... (5 entities in total)
• 機能のキーワード: scf, hormone-growth factor complex, hormone/growth factor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Single-pass type I membrane protein (By similarity). Soluble KIT ligand: Secreted: P21583
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64832.96

構造登録者

Zhang, Z.,Zhang, R.,Joachimiak, A.,Schlessinger, J.,Kong, X. (登録日: 2000-05-05, 公開日: 2000-07-06, 最終更新日: 2024-11-20)

主引用文献

Zhang, Z.,Zhang, R.,Joachimiak, A.,Schlessinger, J.,Kong, X.P.
Crystal structure of human stem cell factor: implication for stem cell factor receptor dimerization and activation.
Proc.Natl.Acad.Sci.USA, 97:7732-7737, 2000

PubMed Abstract: Stem cell factor (SCF) plays important roles in hematopoiesis and the survival, proliferation, and differentiation of mast cells, melanocytes, and germ cells. SCF mediates its biological effects by binding to and activating a receptor tyrosine kinase designated c-kit or SCF receptor. In this report we describe the 2.3-A crystal structure of the functional core of recombinant human SCF. SCF is a noncovalent homodimer composed of two slightly wedged protomers. Each SCF protomer exhibits an antiparallel four-helix bundle fold. Dimerization is mediated by extensive polar and nonpolar interactions between the two protomers with a large buried surface area. Finally, we have identified a hydrophobic crevice and a charged region at the tail of each protomer that functions as a potential receptor-binding site. On the basis of these observations, a model for SCF small middle dotc-kit complex formation and dimerization is proposed.

PubMed: 10884405
DOI: 10.1073/pnas.97.14.7732

実験手法

X-RAY DIFFRACTION (2.3 Å)