1EVO

NMR OBSERVATION OF A NOVEL C-TETRAD

Summary for 1EVO

• Entry DOI: 10.2210/pdb1evo/pdb
• NMR Information: BMRB: 4612
• Descriptor: DNA (5'-D(*TP*GP*GP*GP*CP*GP*GP*T)-3') (1 entity in total)
• Functional Keywords: nmr structure; g-quadruplex; c-tetrad; gggcgg repeat, sv40, dna
• Total number of polymer chains: 4
• Total formula weight: 9994.56

Authors

Patel, P.K.,Bhavesh, N.S.,Hosur, R.V. (deposition date: 2000-04-20, release date: 2000-05-01, Last modification date: 2024-05-22)

Primary citation

Patel, P.K.,Bhavesh, N.S.,Hosur, R.V.
NMR observation of a novel C-tetrad in the structure of the SV40 repeat sequence GGGCGG.
Biochem.Biophys.Res.Commun., 270:967-971, 2000

PubMed Abstract: We report the NMR structure of the DNA sequence d-TGGGCGGT in Na(+) solutions at neutral pH, containing a repeat sequence from SV40 viral genome. The structure is a novel quadruplex incorporating the C-tetrad formed by symmetrical pairing of four Cs via NH(2)&bond;O(2) H-bonds in a plane. The C-tetrad has a wider cavity compared to G-tetrads and stacks well over the adjacent G4-tetrad, but poorly on the G6 tetrad. The quadruplex helix is largely underwound by 8-10 degrees compared to B-DNA except at the C5-G6 step. To our knowledge this is the first report of C-tetrad formation in DNA structures, and would be of significance from the point of view of both structural diversity and specific recognition.

PubMed: 10772934
DOI: 10.1006/bbrc.2000.2479

Experimental method

SOLUTION NMR