1EVM

NMR OBSERVATION OF A-TETRAD

1EVM の概要

• エントリーDOI: 10.2210/pdb1evm/pdb
• NMR情報: BMRB: 4610
• 分子名称: DNA (5'-D(*AP*GP*GP*GP*T)-3') (1 entity in total)
• 機能のキーワード: a-tetrad, g-quadruplex, human telomere, dna
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 6240.24

構造登録者

Patel, P.K.,Koti, A.S.R.,Hosur, R.V. (登録日: 2000-04-20, 公開日: 2000-05-22, 最終更新日: 2024-05-22)

主引用文献

Patel, P.K.,Koti, A.S.,Hosur, R.V.
NMR studies on truncated sequences of human telomeric DNA: observation of a novel A-tetrad.
Nucleic Acids Res., 27:3836-3843, 1999

PubMed Abstract: The structure of the telomeric DNA has been a subject of extensive investigation in recent years due to the realization that it has important functional roles to play in vivo and the observations that truncated telomeric sequences exhibit a great variety of 3D structures in aqueous solutions. In this context, we describe here NMR structural studies on two truncated human telomeric DNA sequences, d-AG(3)T and d-TAG(3)T in solutions containing K(+)ions. The G(3)stretches in both the oligonucleotides were seen to form parallel-stranded quadruplexes. However, the AG(3)segment as a whole, had different structural characteristics. The structure of d-AG(3)T revealed the formation of a novel A-tetrad, which was not seen in d-TAG(3)T. The A's in the tetrad had syn glycosidic conformation as opposed to the anti conformation of the G's in the G-tetrads. The A-tetrad stacked well over the adjacent G-tetrad and the twist angle at this step was smaller in d-AG(3)T than in d-TAG(3)T. These observations are expected to be significant from the point of view of structural diversity and recognition in telomeres.

PubMed: 10481022
DOI: 10.1093/nar/27.19.3836

実験手法

SOLUTION NMR