1EV0

SOLUTION STRUCTURE OF THE MINE TOPOLOGICAL SPECIFICITY DOMAIN

1EV0 の概要

• エントリーDOI: 10.2210/pdb1ev0/pdb
• 分子名称: MINE (1 entity in total)
• 機能のキーワード: mine, topological specificity, cell division, mincd, minicell, cell cycle
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13427.31

構造登録者

King, G.F.,Maciejewski, M.W.,Pan, B.,Mullen, G.P. (登録日: 2000-04-19, 公開日: 2000-11-01, 最終更新日: 2024-05-22)

主引用文献

King, G.F.,Shih, Y.L.,Maciejewski, M.W.,Bains, N.P.,Pan, B.,Rowland, S.L.,Mullen, G.P.,Rothfield, L.I.
Structural basis for the topological specificity function of MinE.
Nat.Struct.Biol., 7:1013-1017, 2000

PubMed Abstract: Correct positioning of the division septum in Escherichia coli depends on the coordinated action of the MinC, MinD and MinE proteins. Topological specificity is conferred on the MinCD division inhibitor by MinE, which counters MinCD activity only in the vicinity of the preferred midcell division site. Here we report the structure of the homodimeric topological specificity domain of Escherichia coli MinE and show that it forms a novel alphabeta sandwich. Structure-directed mutagenesis of conserved surface residues has enabled us to identify a spatially restricted site on the surface of the protein that is critical for the topological specificity function of MinE.

PubMed: 11062554
DOI: 10.1038/80917

実験手法

SOLUTION NMR