1EUV

X-RAY STRUCTURE OF THE C-TERMINAL ULP1 PROTEASE DOMAIN IN COMPLEX WITH SMT3, THE YEAST ORTHOLOG OF SUMO.

1EUV の概要

• エントリーDOI: 10.2210/pdb1euv/pdb
• 分子名称: ULP1 PROTEASE, UBITQUTIN-LIKE PROTEIN SMT3 (3 entities in total)
• 機能のキーワード: sumo hydrolase, ubiquitin-like protease 1, smt3 hydrolase desumoylating enzyme, cysteine protease, sumo processing enzyme, smt3 processing enzyme, nabh4, thiohemiacetal, covalent protease adduct, hydrolase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35611.58

構造登録者

Mossessova, E.,Lima, C.D. (登録日: 2000-04-17, 公開日: 2000-06-07, 最終更新日: 2024-11-13)

主引用文献

Mossessova, E.,Lima, C.D.
Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast.
Mol.Cell, 5:865-876, 2000

PubMed Abstract: Modification of cellular proteins by the ubiquitin-like protein SUMO is essential for nuclear processes and cell cycle progression in yeast. The Ulp1 protease catalyzes two essential functions in the SUMO pathway: (1) processing of full-length SUMO to its mature form and (2) deconjugation of SUMO from targeted proteins. Selective reduction of the proteolytic reaction produced a covalent thiohemiacetal transition state complex between a Ulp1 C-terminal fragment and its cellular substrate Smt3, the yeast SUMO homolog. The Ulp1-Smt3 crystal structure and functional testing of elements within the conserved interface elucidate determinants of SUMO recognition, processing, and deconjugation. Genetic analysis guided by the structure further reveals a regulatory element N-terminal to the proteolytic domain that is required for cell growth in yeast.

PubMed: 10882122
DOI: 10.1016/S1097-2765(00)80326-3

実験手法

X-RAY DIFFRACTION (1.6 Å)