1ESS

STEROID TETHERED DNA, NMR, MINIMIZED AVERAGE STRUCTURE

1ESS の概要

• エントリーDOI: 10.2210/pdb1ess/pdb
• 分子名称: DNA (5'-D(P*TP*GP*GP*AP*GP*CP*TP*G)-3'), DNA (5'-D(P*CP*AP*GP*C)-3'), DNA (5'-D(P*TP*CP*CP*A)-3'), ... (4 entities in total)
• 機能のキーワード: deoxyribonucleic acid, estrone, dna, steroid tethered
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 5509.96

構造登録者

Denisov, A.,Sandstrom, A.,Maltseva, T.,Pyshnyi, D.,Ivanova, E.,Zarytova, V.,Chattopadhyaya, J. (登録日: 1997-06-17, 公開日: 1997-09-17, 最終更新日: 2024-05-22)

主引用文献

Denisov, A.Y.,Sandstrom, A.,Maltseva, T.V.,Pyshnyi, D.V.,Ivanova, E.M.,Zarytova, V.F.,Chattopadhyaya, J.
The NMR structure of estrone (Es)-tethered tandem DNA duplex: [d(5'pCAGCp3')-Es] + [Es-d(5'pTCCA3')]: d(5'pTGGAGCTG3').
J.Biomol.Struct.Dyn., 15:499-516, 1997

PubMed Abstract: The solution structure of an estrone (Es)-tethered tandem DNA duplex consisting of two Es-tethered tetranucleotides and a target octameric DNA sequence is reported. The structure of this Es-tethered tandem duplex has been compared with a corresponding natural tandem duplex without estrones. The Tm of the 3'-Es-tethered tetranucleotide part of the tandem duplex increases by 5 degrees C, whereas the Tm of the 5'-Es-tethered tetranucleotide part increases by 7 degrees C, compared with the corresponding natural counterpart. The NMR structures of both the Es-tethered tandem duplex and the natural counterpart have been based on 24 experimental NMR constraints per residue. Despite the fact that there is considerable distortion at the junction of two Es-tethered tetranucleotides in the major groove of the Es-tethered DNA duplex compared to the natural counterpart, both duplexes do take up B-type DNA structures. It is likely that the spatial proximity of two Es residues, and the resulting hydrophobic interaction between them might be responsible for the increase of the thermal stability of the Es-tethered tandem duplex in comparison with the natural counterpart.

PubMed: 9439997

実験手法

SOLUTION NMR