1ESH

THE SOLUTION STRUCTURE OF THE STEM LOOP C 5'AUA3' TRILOOP OF BROME MOSAIC VIRUS (+) STRAND RNA

1ESH の概要

• エントリーDOI: 10.2210/pdb1esh/pdb
• 分子名称: RNA (5'-R(*GP*GP*UP*GP*CP*AP*UP*AP*GP*CP*AP*CP*C)-3') (1 entity in total)
• 機能のキーワード: rna, triloop, stem-loop, brome mosaic virus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4156.54

構造登録者

Kim, C.-H.,Kao, C.C.,Tinoco Jr., I. (登録日: 2000-04-10, 公開日: 2000-04-24, 最終更新日: 2024-05-22)

主引用文献

Kim, C.H.,Kao, C.C.,Tinoco Jr., I.
RNA motifs that determine specificity between a viral replicase and its promoter.
Nat.Struct.Biol., 7:415-423, 2000

PubMed Abstract: The 3' end of brome mosaic virus RNA contains a tRNA-like sequence that directs its RNA synthesis. A stem loop structure in this sequence, stem loop C (SLC), was investigated using NMR, and correlated with its ability to direct RNA synthesis by its replicase. SLC consists of two discrete domains, a flexible stem with an internal loop and a rigid stem containing a 5'-AUA-3' triloop. Efficient RNA synthesis requires the sequence on only one side of the flexible stem and a specific compact conformation of the triloop. A high resolution structure of the triloop places the 5' adenine out in solution, and the 3' adenine within the triloop, held tightly through stacking and unusual hydrogen bonds. This high resolution structure of an RNA promoter from a (+)-strand RNA virus provides new insights into how the RNA-dependent RNA polymerase binds to the RNA to initiate synthesis.

PubMed: 10802741
DOI: 10.1038/75202

実験手法

SOLUTION NMR