1ESG

RESTRICTION ENDONUCLEASE BAMHI BOUND TO A NON-SPECIFIC DNA.

1ESG の概要

• エントリーDOI: 10.2210/pdb1esg/pdb
• 関連するPDBエントリー: 1bam 1bhm 2bam 3bam
• 分子名称: DNA (5'-D(*TP*GP*GP*AP*TP*TP*CP*A)-3'), DNA (5'-D(*TP*GP*AP*AP*TP*CP*CP*A)-3'), TYPE II RESTRICTION ENZYME BAMHI, ... (4 entities in total)
• 機能のキーワード: non-specific dna-protein complex., hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Bacillus amyloliquefaciens; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 54056.84

構造登録者

Viadiu, H.,Aggarwal, A.K. (登録日: 2000-04-09, 公開日: 2000-05-31, 最終更新日: 2024-02-07)

主引用文献

Viadiu, H.,Aggarwal, A.K.
Structure of BamHI bound to nonspecific DNA: a model for DNA sliding.
Mol.Cell, 5:889-895, 2000

PubMed Abstract: The central problem faced by DNA binding proteins is how to select the correct DNA sequence from the sea of nonspecific sequences in a cell. The problem is particularly acute for bacterial restriction enzymes because cleavage at an incorrect DNA site could be lethal. To understand the basis of this selectivity, we report here the crystal structure of endonuclease BamHI bound to noncognate DNA. We show that, despite only a single base pair change in the recognition sequence, the enzyme adopts an open configuration that is on the pathway between free and specifically bound forms of the enzyme. Surprisingly, the DNA drops out of the binding cleft with a total loss of base-specific and backbone contacts. Taken together, the structure provides a remarkable snapshot of an enzyme poised for linear diffusion (rather than cleavage) along the DNA.

PubMed: 10882125
DOI: 10.1016/S1097-2765(00)80329-9

実験手法

X-RAY DIFFRACTION (1.9 Å)