1ES6

CRYSTAL STRUCTURE OF THE MATRIX PROTEIN OF EBOLA VIRUS

1ES6 の概要

• エントリーDOI: 10.2210/pdb1es6/pdb
• 分子名称: MATRIX PROTEIN VP40 (2 entities in total)
• 機能のキーワード: beta sandwich, anti-parallel strands, beta sheet, helix, viral protein
• 由来する生物種: Ebola virus sp.
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31860.82

構造登録者

Dessen, A.,Volchkov, V.,Dolnik, O.,Klenk, H.-D.,Weissenhorn, W. (登録日: 2000-04-07, 公開日: 2000-08-30, 最終更新日: 2024-02-07)

主引用文献

Dessen, A.,Volchkov, V.,Dolnik, O.,Klenk, H.D.,Weissenhorn, W.
Crystal structure of the matrix protein VP40 from Ebola virus.
EMBO J., 19:4228-4236, 2000

PubMed Abstract: Ebola virus maturation occurs at the plasma membrane of infected cells and involves the clustering of the viral matrix protein VP40 at the assembly site as well as its interaction with the lipid bilayer. Here we report the X-ray crystal structure of VP40 from Ebola virus at 2.0 A resolution. The crystal structure reveals that Ebola virus VP40 is topologically distinct from all other known viral matrix proteins, consisting of two domains with unique folds, connected by a flexible linker. The C-terminal domain, which is absolutely required for membrane binding, contains large hydrophobic patches that may be involved in the interaction with lipid bilayers. Likewise, a highly basic region is shared between the two domains. The crystal structure reveals how the molecule may be able to switch from a monomeric conformation to a hexameric form, as observed in vitro. Its implications for the assembly process are discussed.

PubMed: 10944105
DOI: 10.1093/emboj/19.16.4228

実験手法

X-RAY DIFFRACTION (2 Å)