1ERZ

CRYSTAL STRUCTURE OF N-CARBAMYL-D-AMINO ACID AMIDOHYDROLASE WITH A NOVEL CATALYTIC FRAMEWORK COMMON TO AMIDOHYDROLASES

1ERZ の概要

• エントリーDOI: 10.2210/pdb1erz/pdb
• 分子名称: N-CARBAMYL-D-AMINO ACID AMIDOHYDROLASE (2 entities in total)
• 機能のキーワード: four-layer sandwich, hydrolase
• 由来する生物種: Agrobacterium sp.
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 68398.19

構造登録者

Nakai, T.,Hasegawa, T.,Yamashita, E.,Yamamoto, M.,Kumasaka, T.,Ueki, T.,Nanba, H.,Ikenaka, Y.,Takahashi, S.,Sato, M.,Tsukihara, T. (登録日: 2000-04-06, 公開日: 2001-04-06, 最終更新日: 2024-02-07)

主引用文献

Nakai, T.,Hasegawa, T.,Yamashita, E.,Yamamoto, M.,Kumasaka, T.,Ueki, T.,Nanba, H.,Ikenaka, Y.,Takahashi, S.,Sato, M.,Tsukihara, T.
Crystal structure of N-carbamyl-D-amino acid amidohydrolase with a novel catalytic framework common to amidohydrolases.
Structure Fold.Des., 8:729-737, 2000

PubMed Abstract: N-carbamyl-D-amino acid amidohydrolase (DCase) catalyzes the hydrolysis of N-carbamyl-D-amino acids to the corresponding D-amino acids, which are useful intermediates in the preparation of beta-lactam antibiotics. To understand the catalytic mechanism of N-carbamyl-D-amino acid hydrolysis, the substrate specificity and thermostability of the enzyme, we have determined the structure of DCase from Agrobacterium sp. strain KNK712.

PubMed: 10903946
DOI: 10.1016/S0969-2126(00)00160-X

実験手法

X-RAY DIFFRACTION (1.7 Å)