1EPX

CRYSTAL STRUCTURE ANALYSIS OF ALDOLASE FROM L. MEXICANA

1EPX の概要

• エントリーDOI: 10.2210/pdb1epx/pdb
• 分子名称: FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE (2 entities in total)
• 機能のキーワード: beta barrel, pts signal, aldolase, leishmania, fructose-1, 6-bisphosphate, lyase
• 由来する生物種: Leishmania mexicana
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 162894.16

構造登録者

Chudzik, D.M.,Michels, P.A.,de Walque, S.,Hol, W.G.J. (登録日: 2000-03-29, 公開日: 2000-07-13, 最終更新日: 2024-04-03)

主引用文献

Chudzik, D.M.,Michels, P.A.,de Walque, S.,Hol, W.G.
Structures of type 2 peroxisomal targeting signals in two trypanosomatid aldolases.
J.Mol.Biol., 300:697-707, 2000

PubMed Abstract: Trypanosomatids, unicellular organisms responsible for several global diseases, contain unique organelles called glycosomes in which the first seven glycolytic enzymes are sequestered. We report the crystal structures of glycosomal fructose-1,6-bisphosphate aldolase from two major tropical pathogens, Trypanosoma brucei and Leishmania mexicana, the causative agents of African sleeping sickness and one form of leishmaniasis, respectively. Unlike mammalian aldolases, the T. brucei and L. mexicana aldolases contain nonameric N-terminal type 2 peroxisomal targeting signals (PTS2s) to direct their import into the glycosome. In both tetrameric trypanosomatid aldolases, the PTS2s from two different subunits form two closely intertwined structures. These "PTS2 dimers", which have very similar conformations in the two aldolase structures, are the first reported conformations of a glycosomal or peroxisomal PTS2, and provide opportunities for the design of trypanocidal compounds.

PubMed: 10891264
DOI: 10.1006/jmbi.2000.3910

実験手法

X-RAY DIFFRACTION (1.8 Å)