1EPI

THREE-DIMENSIONAL NUCLEAR MAGNETIC RESONANCE STRUCTURES OF MOUSE EPIDERMAL GROWTH FACTOR IN ACIDIC AND PHYSIOLOGICAL PH SOLUTIONS

1EPI の概要

• エントリーDOI: 10.2210/pdb1epi/pdb
• 分子名称: EPIDERMAL GROWTH FACTOR (1 entity in total)
• 機能のキーワード: growth factor
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P01132
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6050.72

構造登録者

Kohda, D.,Inagaki, F. (登録日: 1992-03-24, 公開日: 1994-01-31, 最終更新日: 2024-10-30)

主引用文献

Kohda, D.,Inagaki, F.
Three-dimensional nuclear magnetic resonance structures of mouse epidermal growth factor in acidic and physiological pH solutions.
Biochemistry, 31:11928-11939, 1992

PubMed Abstract: The three-dimensional structures of epidermal growth factors (EGF) previously reported were all in acidic solutions (pH 2.0-3.2), at which pHs EGF cannot bind to the receptor. Here we studied the structure of mouse EGF at pH 6.8, where EGF is physiologically active, and compared it with the structure at pH 2.0 by CD and NMR. From pH dependence of CD spectra and a comparison between the chemical shifts of the proton resonances at pH 6.8 and 2.0, the conformations at two pHs were found to be nearly identical except for the C-terminal tail region. The three-dimensional structures at pH 6.8 and 2.0 were determined independently by a combination of two-dimensional 1H NMR and stimulated annealing calculations using the program XPLOR. The calculations were based on 261 distance constraints at pH 6.8 and 355 distance and 24 torsion angle constraints at pH 2.0. The conformational difference of the C-terminal domain (residues 33-50) was detected between the two structures, which were supported by CD and the chemical shift comparison. The positions of the side chains of Leu47, Arg48, Trp49, and Trp50 are changed probably by the effect of the deprotonation of Asp46. Considering the fact that Leu47 is essential in EGF binding to the receptor, this conformational difference may be important in receptor recognition.

PubMed: 1445923
DOI: 10.1021/bi00162a036

実験手法

SOLUTION NMR