1EMZ

SOLUTION STRUCTURE OF FRAGMENT (350-370) OF THE TRANSMEMBRANE DOMAIN OF HEPATITIS C ENVELOPE GLYCOPROTEIN E1

1EMZ の概要

• エントリーDOI: 10.2210/pdb1emz/pdb
• 分子名称: ENVELOPE GLYCOPROTEIN E1 (1 entity in total)
• 機能のキーワード: transmembrane domain, envelope protein e1, hepatitis c virus, viral protein
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2237.58

構造登録者

Op De Beeck, A.,Montserret, R.,Duvet, S.,Cocquerel, L.,Cacan, R.,Barberot, B.,Le Maire, M.,Penin, F.,Dubuisson, J. (登録日: 2000-03-20, 公開日: 2000-04-05, 最終更新日: 2024-05-22)

主引用文献

Op De Beeck, A.,Montserret, R.,Duvet, S.,Cocquerel, L.,Cacan, R.,Barberot, B.,Le Maire, M.,Penin, F.,Dubuisson, J.
The transmembrane domains of hepatitis C virus envelope glycoproteins E1 and E2 play a major role in heterodimerization.
J.Biol.Chem., 275:31428-31437, 2000

PubMed Abstract: Oligomerization of viral envelope proteins is essential to control virus assembly and fusion. The transmembrane domains (TMDs) of hepatitis C virus envelope glycoproteins E1 and E2 have been shown to play multiple functions during the biogenesis of E1E2 heterodimer. This makes them very unique among known transmembrane sequences. In this report, we used alanine scanning insertion mutagenesis in the TMDs of E1 and E2 to examine their role in the assembly of E1E2 heterodimer. Alanine insertion within the center of the TMDs of E1 or E2 or in the N-terminal part of the TMD of E1 dramatically reduced heterodimerization, demonstrating the essential role played by these domains in the assembly of hepatitis C virus envelope glycoproteins. To better understand the alanine scanning data obtained for the TMD of E1 which contains GXXXG motifs, we analyzed by circular dichroism and nuclear magnetic resonance the three-dimensional structure of the E1-(350-370) peptide encompassing the N-terminal sequence of the TMD of E1 involved in heterodimerization. Alanine scanning results and the three-dimensional molecular model we obtained provide the first framework for a molecular level understanding of the mechanism of hepatitis C virus envelope glycoprotein heterodimerization.

PubMed: 10807921
DOI: 10.1074/jbc.M003003200

実験手法

SOLUTION NMR