1EMA

GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA

1EMA の概要

• エントリーDOI: 10.2210/pdb1ema/pdb
• 分子名称: GREEN FLUORESCENT PROTEIN (2 entities in total)
• 機能のキーワード: beta-barrel, autocatalytic, fluorophore, bioluminescense fluorescent protein, fluorescent protein
• 由来する生物種: Aequorea victoria
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 27226.75

構造登録者

Ormo, M.,Remington, S.J. (登録日: 1996-08-01, 公開日: 1996-11-08, 最終更新日: 2026-03-18)

主引用文献

Ormo, M.,Cubitt, A.B.,Kallio, K.,Gross, L.A.,Tsien, R.Y.,Remington, S.J.
Crystal structure of the Aequorea victoria green fluorescent protein.
Science, 273:1392-1395, 1996

PubMed Abstract: The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequorea victoria has generated intense interest as a marker for gene expression and localization of gene products. The chromophore, resulting from the spontaneous cyclization and oxidation of the sequence -Ser65 (or Thr65)-Tyr66-Gly67-, requires the native protein fold for both formation and fluorescence emission. The structure of Thr65 GFP has been determined at 1.9 angstrom resolution. The protein fold consists of an 11-stranded beta barrel with a coaxial helix, with the chromophore forming from the central helix. Directed mutagenesis of one residue adjacent to the chromophore, Thr203, to Tyr or His results in significantly red-shifted excitation and emission maxima.

PubMed: 8703075

実験手法

X-RAY DIFFRACTION (1.9 Å)