1EKE

CRYSTAL STRUCTURE OF CLASS II RIBONUCLEASE H (RNASE HII) WITH MES LIGAND

1EKE の概要

• エントリーDOI: 10.2210/pdb1eke/pdb
• 分子名称: RIBONUCLEASE HII, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID (3 entities in total)
• 機能のキーワード: nuclease, endonuclease, structural genomics, bsgc structure funded by nih, protein structure initiative, psi, berkeley structural genomics center, hydrolase
• 由来する生物種: Methanocaldococcus jannaschii
• 細胞内の位置: Cytoplasm (Potential): Q57599
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53777.69

構造登録者

Lai, L.H.,Yokota, H.,Hung, L.W.,Kim, R.,Kim, S.H.,Berkeley Structural Genomics Center (BSGC) (登録日: 2000-03-07, 公開日: 2000-09-13, 最終更新日: 2024-11-06)

主引用文献

Lai, L.,Yokota, H.,Hung, L.W.,Kim, R.,Kim, S.H.
Crystal structure of archaeal RNase HII: a homologue of human major RNase H
Structure, 8:897-904, 2000

PubMed Abstract: RNases H are present in all organisms and cleave RNAs in RNA/DNA hybrids. There are two major types of RNases H that have little similarity in sequence, size and specificity. The structure of RNase HI, the smaller enzyme and most abundant in bacteria, has been extensively studied. However, no structural information is available for the larger RNase H, which is most abundant in eukaryotes and archaea. Mammalian RNase H participates in DNA replication, removal of the Okazaki fragments and possibly DNA repair.

PubMed: 10997908
DOI: 10.1016/S0969-2126(00)00179-9

実験手法

X-RAY DIFFRACTION (2 Å)