1EK4

BETA-KETOACYL [ACYL CARRIER PROTEIN] SYNTHASE I IN COMPLEX WITH DODECANOIC ACID TO 1.85 RESOLUTION

1EK4 の概要

• エントリーDOI: 10.2210/pdb1ek4/pdb
• 関連するPDBエントリー: 1dd8
• 分子名称: BETA-KETOACYL [ACYL CARRIER PROTEIN] SYNTHASE I, LAURIC ACID (3 entities in total)
• 機能のキーワード: fatty acid synthase, thiolase fold, claisen condensation, fatty acid substrate complex, transferase
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: P0A953
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 177096.10

構造登録者

Olsen, J.G.,Kadziola, A.,Siggaard-Andersen, M.,von Wettstein-Knowles, P.,Larsen, S. (登録日: 2000-03-06, 公開日: 2001-04-11, 最終更新日: 2024-10-30)

主引用文献

Olsen, J.G.,Kadziola, A.,von Wettstein-Knowles, P.,Siggaard-Andersen, M.,Larsen, S.
Structures of beta-ketoacyl-acyl carrier protein synthase I complexed with fatty acids elucidate its catalytic machinery.
Structure, 9:233-243, 2001

PubMed Abstract: beta-ketoacyl-acyl carrier protein synthase (KAS) I is vital for the construction of the unsaturated fatty acid carbon skeletons characterizing E. coli membrane lipids. The new carbon-carbon bonds are created by KAS I in a Claisen condensation performed in a three-step enzymatic reaction. KAS I belongs to the thiolase fold enzymes, of which structures are known for five other enzymes.

PubMed: 11286890
DOI: 10.1016/S0969-2126(01)00583-4

実験手法

X-RAY DIFFRACTION (1.85 Å)