1EJY

MOUSE IMPORTIN ALPHA-NUCLEOPLASMIN NLS PEPTIDE COMPLEX

1EJY の概要

• エントリーDOI: 10.2210/pdb1ejy/pdb
• 関連するPDBエントリー: 1BK6 1EJL 1IAL
• 分子名称: NUCLEOPLASMIN NLS PEPTIDE, IMPORTIN ALPHA (2 entities in total)
• 機能のキーワード: importin aplpha/karyopherin alpha; nuclear localization sequence (nls) recognition; nucleoplasmin, protein binding, hydrolase-peptide complex, hydrolase/peptide
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51633.81

構造登録者

Fontes, M.R.,Teh, T.,Kobe, B. (登録日: 2000-03-05, 公開日: 2000-04-24, 最終更新日: 2024-02-07)

主引用文献

Fontes, M.R.,Teh, T.,Kobe, B.
Structural basis of recognition of monopartite and bipartite nuclear localization sequences by mammalian importin-alpha.
J.Mol.Biol., 297:1183-1194, 2000

PubMed Abstract: Importin-alpha is the nuclear import receptor that recognizes cargo proteins which contain classical monopartite and bipartite nuclear localization sequences (NLSs), and facilitates their transport into the nucleus. To determine the structural basis of the recognition of the two classes of NLSs by mammalian importin-alpha, we co-crystallized an N-terminally truncated mouse receptor protein with peptides corresponding to the monopartite NLS from the simian virus 40 (SV40) large T-antigen, and the bipartite NLS from nucleoplasmin. We show that the monopartite SV40 large T-antigen NLS binds to two binding sites on the receptor, similar to what was observed in yeast importin-alpha. The nucleoplasmin NLS-importin-alpha complex shows, for the first time, the mode of binding of bipartite NLSs to the receptor. The two basic clusters in the NLS occupy the two binding sites used by the monopartite NLS, while the sequence linking the two basic clusters is poorly ordered, consistent with its tolerance to mutations. The structures explain the structural basis for binding of diverse NLSs to the sole receptor protein.

PubMed: 10764582
DOI: 10.1006/jmbi.2000.3642

実験手法

X-RAY DIFFRACTION (2.9 Å)