1EJA

STRUCTURE OF PORCINE TRYPSIN COMPLEXED WITH BDELLASTASIN, AN ANTISTASIN-TYPE INHIBITOR

1EJA の概要

• エントリーDOI: 10.2210/pdb1eja/pdb
• 関連するPDBエントリー: 1C9P 1C9T
• 分子名称: TRYPSIN, BDELLASTASIN, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: complex (hydrolase-inhibitor), hydrolase, inhibitor, bdellastasin, antistasin, trypsin, hydrolase-inhibitor complex, hydrolase/inhibitor
• 由来する生物種: Hirudo medicinalis (medicinal leech); 詳細
• 細胞内の位置: Secreted, extracellular space: P00761; Secreted: P82107
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29867.69

構造登録者

Rester, U.,Moser, M.,Huber, R.,Bode, W. (登録日: 2000-03-02, 公開日: 2001-03-02, 最終更新日: 2024-10-30)

主引用文献

Rester, U.,Moser, M.,Huber, R.,Bode, W.
L-Isoaspartate 115 of porcine beta-trypsin promotes crystallization of its complex with bdellastasin.
Acta Crystallogr.,Sect.D, 56:581-588, 2000

PubMed Abstract: Bdellastasin is a 59-amino-acid, cysteine-rich, antistasin-type inhibitor of sperm acrosin, plasmin and trypsin, isolated from the medicinal leech Hirudo medicinalis. The complex formed between bdellastasin and porcine beta-trypsin has previously been crystallized in the presence of PEG in a tetragonal crystal form of space group P4(3)2(1)2 and has now been found to crystallize under high-salt conditions in the enantiomorphic space group P4(1)2(1)2. These structures have been solved and refined to 2.8 and 2.7 A resolution, respectively. Bdellastasin turns out to have an antistasin-like fold exhibiting a bis-domainal structure. In the second new crystal form, the flexible N-terminal subdomain is rotated with respect to the C--terminal subdomain by about 90 degrees, fitting into a cavity formed by symmetry-related trypsin molecules. The canonical inhibitor-proteinase interaction is restricted to the primary binding loop comprising residues Leu31-Lys36 of bdellastasin. During the refinement, a bound sodium ion occupying the calcium-binding site of the porcine beta-trypsin component was discovered. This sodium ion is coordinated in a tetragonal-pyramidal manner, with the geometry of the enclosing loop slightly changed compared with the loop in the presence of calcium. In the crystal form of space group P4(3)2(1)2, the electron density for residue 115 of porcine beta-trypsin clearly indicates the presence of a beta-isomerized L-aspartic acid, which is placed in spatial proximity to segment Thr144--Gly148 of a symmetry-related trypsin molecule. This is the first structurally observed example of an L-isoaspartate in beta--trypsin originating from Asn. A comparison with other known crystal structures of porcine beta-trypsin-macromolecular inhibitor complexes suggests that the deamidation, isomerization and racemization of Asn115 is the key step in crystallization.

PubMed: 10771427
DOI: 10.1107/S0907444900003048

実験手法

X-RAY DIFFRACTION (2.7 Å)