1EJ0

FTSJ RNA METHYLTRANSFERASE COMPLEXED WITH S-ADENOSYLMETHIONINE, MERCURY DERIVATIVE

1EJ0 の概要

• エントリーDOI: 10.2210/pdb1ej0/pdb
• 関連するPDBエントリー: 1EIZ
• 分子名称: FTSJ, MERCURY (II) ION, S-ADENOSYLMETHIONINE, ... (4 entities in total)
• 機能のキーワード: ftsj, methyltransferase, adomet, adenosyl methionine, heat shock proteins, 23s ribosomal rna, transferase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20736.82

構造登録者

Bugl, H.,Fauman, E.B.,Staker, B.L.,Zheng, F.,Kushner, S.R.,Saper, M.A.,Bardwell, J.C.A.,Jakob, U. (登録日: 2000-02-29, 公開日: 2000-08-30, 最終更新日: 2023-08-09)

主引用文献

Bugl, H.,Fauman, E.B.,Staker, B.L.,Zheng, F.,Kushner, S.R.,Saper, M.A.,Bardwell, J.C.,Jakob, U.
RNA methylation under heat shock control.
Mol.Cell, 6:349-360, 2000

PubMed Abstract: Structural, biochemical, and genetic techniques were applied to investigate the function of FtsJ, a recently identified heat shock protein. FtsJ is well conserved, from bacteria to humans. The 1.5 A crystal structure of FtsJ in complex with its cofactor S-adenosylmethionine revealed that FtsJ has a methyltransferase fold. The molecular surface of FtsJ exposes a putative nucleic acid binding groove composed of highly conserved, positively charged residues. Substrate analysis showed that FtsJ methylates 23S rRNA within 50S ribosomal subunits in vitro and in vivo. Null mutations in ftsJ show a dramatically altered ribosome profile, a severe growth disadvantage, and a temperature-sensitive phenotype. Our results reveal an unexpected link between the heat shock response and RNA metabolism.

PubMed: 10983982
DOI: 10.1016/S1097-2765(00)00035-6

実験手法

X-RAY DIFFRACTION (1.5 Å)