1EIW

Solution structure of hypothetical protein MTH538 from Methanobacterium thermoautotrophicum

1EIW の概要

• エントリーDOI: 10.2210/pdb1eiw/pdb
• 分子名称: HYPOTHETICAL PROTEIN MTH538 (1 entity in total)
• 機能のキーワード: chey-like fold, flavodoxin-like fold, (a/b)5 doubly wound fold, parallel beta sheet, structural genomics, psi, protein structure initiative, northeast structural genomics consortium, nesg
• 由来する生物種: Methanothermobacter thermautotrophicus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12395.94

構造登録者

Cort, J.R.,Arrowsmith, C.H.,Kennedy, M.A.,Northeast Structural Genomics Consortium (NESG) (登録日: 2000-02-29, 公開日: 2000-09-06, 最終更新日: 2024-05-22)

主引用文献

Cort, J.R.,Yee, A.,Edwards, A.M.,Arrowsmith, C.H.,Kennedy, M.A.
Structure-based functional classification of hypothetical protein MTH538 from Methanobacterium thermoautotrophicum.
J.Mol.Biol., 302:189-203, 2000

PubMed Abstract: The structure of MTH538, a previously uncharacterized hypothetical protein from Methanobacterium thermoautotrophicum, has been determined by NMR spectroscopy. MTH538 is one of numerous structural genomics targets selected in a genome-wide survey of uncharacterized sequences from this organism. MTH538 is a so-called singleton, a sequence not closely related to any other (known) sequences. The structure of MTH538 closely resembles the known structures of receiver domains from two component response regulator systems, such as CheY, and is similar to the structures of flavodoxins and GTP-binding proteins. Tests on MTH538 for characteristic activities of CheY and flavodoxin were negative. MTH538 did not become phosphorylated in the presence of acetyl phosphate and Mg(2+), although it appeared to bind Mg(2+). MTH538 also did not bind flavin mononucleotide (FMN) or coenzyme F(420). Nevertheless, sequence and structure parallels between MTH538/CheY and two families of ATPase/phosphatase proteins suggest that MTH538 may have a role in a phosphorylation-independent two-component response regulator system.

PubMed: 10964569
DOI: 10.1006/jmbi.2000.4052

実験手法

SOLUTION NMR