1EIG

SOLUTION STRUCTURE OF THE HUMAN CHEMOKINE EOTAXIN-2

1EIG の概要

• エントリーDOI: 10.2210/pdb1eig/pdb
• 関連するPDBエントリー: 1EIH
• NMR情報: BMRB: 4590
• 分子名称: EOTAXIN-2 (1 entity in total)
• 機能のキーワード: chemokine, chemotactic cytokine, eosinophil chemoattractant, cytokine
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8323.78

構造登録者

Mayer, K.L.,Stone, M.J. (登録日: 2000-02-25, 公開日: 2000-12-06, 最終更新日: 2024-11-20)

主引用文献

Mayer, K.L.,Stone, M.J.
NMR solution structure and receptor peptide binding of the CC chemokine eotaxin-2.
Biochemistry, 39:8382-8395, 2000

PubMed Abstract: The human CC chemokine eotaxin-2 is a specific agonist for the chemokine receptor CCR3 and may play a role in the recruitment of eosinophils in allergic diseases and parasitic infections. We report the solution structure of eotaxin-2 determined using heteronuclear and triple resonance NMR methods. A family of 20 structures was calculated by hybrid distance geometry-simulated annealing from 854 NOE distance restraints, 48 dihedral angle restraints, and 12 hydrogen bond restraints. The structure of eotaxin-2 (73 amino acid residues) consists of a helical turn (residues 17-20) followed by a 3-stranded antiparallel beta-sheet (residues 22-26, 37-41, and 44-49) and an alpha-helix (residues 54-66). The N-loop (residues 9-16) is packed against both the sheet and the helix with the two conserved disulfide bonds tethering the N-terminal/N-loop region to the beta-sheet. The average backbone and heavy atom rmsd values of the 20 structures (residues 7-66) are 0.52 and 1.13 A, respectively. A linear peptide corresponding to the N-terminal region of CCR3 binds to eotaxin-2, inducing concentration-dependent chemical shift changes or line broadening of many residues. The distribution of these residues suggests that the peptide binds into an extended groove located at the interface between the N-loop and the beta2-beta3 hairpin. The receptor peptide may also interact with the N-terminus of the chemokine and part of the alpha-helix. Comparison of the eotaxin-2 structure with those of related chemokines indicates several structural features that may contribute to receptor specificity.

PubMed: 10913244
DOI: 10.1021/bi000523j

実験手法

SOLUTION NMR