1EGX

SOLUTION STRUCTURE OF THE ENA-VASP HOMOLOGY 1 (EVH1) DOMAIN OF HUMAN VASODILATOR-STIMULATED PHOSPHOPROTEIN (VASP)

1EGX の概要

• エントリーDOI: 10.2210/pdb1egx/pdb
• 関連するPDBエントリー: 1evh 1qc6
• 分子名称: VASODILATOR-STIMULATED PHOSPHOPROTEIN (1 entity in total)
• 機能のキーワード: evh1, vasp-ena, poly-proline-binding domain, signaling protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P50552
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12746.36

構造登録者

Ball, L.,Kuhne, R.,Hoffmann, B.,Hafner, A.,Schmieder, P.,Volkmer-Engert, R.,Hof, M.,Wahl, M.,Schneider-Mergener, J.,Walter, U.,Oschkinat, H.,Jarchau, T. (登録日: 2000-02-17, 公開日: 2000-09-20, 最終更新日: 2024-05-01)

主引用文献

Ball, L.J.,Kuhne, R.,Hoffmann, B.,Hafner, A.,Schmieder, P.,Volkmer-Engert, R.,Hof, M.,Wahl, M.,Schneider-Mergener, J.,Walter, U.,Oschkinat, H.,Jarchau, T.
Dual epitope recognition by the VASP EVH1 domain modulates polyproline ligand specificity and binding affinity.
EMBO J., 19:4903-4914, 2000

PubMed Abstract: The Ena-VASP family of proteins act as molecular adaptors linking the cytoskeletal system to signal transduction pathways. Their N-terminal EVH1 domains use groups of exposed aromatic residues to specifically recognize 'FPPPP' motifs found in the mammalian zyxin and vinculin proteins, and ActA protein of the intracellular bacterium Listeria monocytogenes. Here, evidence is provided that the affinities of these EVH1-peptide interactions are strongly dependent on the recognition of residues flanking the core FPPPP motifs. Determination of the VASP EVH1 domain solution structure, together with peptide library screening, measurement of individual K(d)s by fluorescence titration, and NMR chemical shift mapping, revealed a second affinity-determining epitope present in all four ActA EVH1-binding motifs. The epitope was shown to interact with a complementary hydrophobic site on the EVH1 surface and to increase strongly the affinity of ActA for EVH1 domains. We propose that this epitope, which is absent in the sequences of the native EVH1-interaction partners zyxin and vinculin, may provide the pathogen with an advantage when competing for the recruitment of the host VASP and Mena proteins in the infected cell.

PubMed: 10990454
DOI: 10.1093/emboj/19.18.4903

実験手法

SOLUTION NMR