1EGW

CRYSTAL STRUCTURE OF MEF2A CORE BOUND TO DNA

1EGW の概要

• エントリーDOI: 10.2210/pdb1egw/pdb
• 分子名称: DNA (5'-D(*AP*AP*AP*GP*CP*TP*AP*TP*TP*AP*TP*TP*AP*GP*CP*TP*T)-3'), DNA (5'-D(*TP*AP*AP*GP*CP*TP*AP*AP*TP*AP*AP*TP*AP*GP*CP*TP*T)-3'), MADS BOX TRANSCRIPTION ENHANCER FACTOR 2, POLYPEPTIDE A, ... (4 entities in total)
• 機能のキーワード: mads-box transcription factor, dna-protein complex, transcription/dna, transcription-dna complex
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : Q02078
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 57442.24

構造登録者

Santelli, E.,Richmond, T.J. (登録日: 2000-02-17, 公開日: 2000-03-20, 最終更新日: 2024-02-07)

主引用文献

Santelli, E.,Richmond, T.J.
Crystal structure of MEF2A core bound to DNA at 1.5 A resolution.
J.Mol.Biol., 297:437-449, 2000

PubMed Abstract: Members of the myocyte enhancer factor-2 (MEF2) family of transcription factors bind to and activate transcription through A+T-rich DNA sequences found primarily, but not exclusively, in the promoters of muscle-specific genes. Their importance has been established for myogenic development and in activation of the immediate-early gene, c-jun, and recently further functional roles in the immune system have emerged. The MEF2 factors belong to the MADS-box superfamily, sharing homology in a 58 amino acid domain that mediates DNA binding and dimerization. The structures of two MADS-box proteins, SRF and MCM1, bound to their cognate DNA have been previously reported and shown to share extensive similarity in their mode of DNA binding. We have solved the structure of MEF2A 2-78 bound to its DNA consensus sequence at 1.5 A resolution. It reveals how the absence of amino acids N-terminal to the MADS-box contributes to the DNA binding properties of MEF2 proteins and shows that the MEF domain C-terminal to the MADS-box adopts a conformation considerably different from the same region in SRF and MCM1.

PubMed: 10715212
DOI: 10.1006/jmbi.2000.3568

実験手法

X-RAY DIFFRACTION (1.5 Å)