1EFK

STRUCTURE OF HUMAN MALIC ENZYME IN COMPLEX WITH KETOMALONATE

Summary for 1EFK

• Entry DOI: 10.2210/pdb1efk/pdb
• Related: 1DO8 1EFL 1QR6
• Descriptor: MALIC ENZYME, MAGNESIUM ION, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ... (5 entities in total)
• Functional Keywords: malic enzyme, closed form, quaternary complex, oxidoreductase
• Biological source: Homo sapiens (human)
• Cellular location: Mitochondrion matrix: P23368
• Total number of polymer chains: 4
• Total formula weight: 270776.30

Authors

Yang, Z.,Floyd, D.L.,Loeber, G.,Tong, L. (deposition date: 2000-02-09, release date: 2000-03-08, Last modification date: 2011-07-13)

Primary citation

Yang, Z.,Floyd, D.L.,Loeber, G.,Tong, L.
Structure of a closed form of human malic enzyme and implications for catalytic mechanism.
Nat.Struct.Biol., 7:251-257, 2000

PubMed Abstract: Malic enzymes are widely distributed in nature and have many biological functions. The crystal structure of human mitochondrial NAD(P)+-dependent malic enzyme in a quaternary complex with NAD+, Mn++ and oxalate has been determined at 2.2 A resolution. The structures of the quaternary complex with NAD+, Mg++, tartronate or ketomalonate have been determined at 2.6 A resolution. The structures show the enzyme in a closed form in these complexes and reveal the binding modes of the cation and the inhibitors. The divalent cation is coordinated in an octahedral fashion by six ligating oxygens, two from the substrate/inhibitor, three from Glu 255, Asp 256 and Asp 279 of the enzyme, and one from a water molecule. The structural information has significant implications for the catalytic mechanism of malic enzymes and identifies Tyr 112 and Lys 183 as possible catalytic residues. Changes in tetramer organization of the enzyme are also observed in these complexes, which might be relevant for its cooperative behavior and allosteric control.

PubMed: 10700286
DOI: 10.1038/73378

Experimental method

X-RAY DIFFRACTION (2.6 Å)