1EFD

PERIPLASMIC FERRIC SIDEROPHORE BINDING PROTEIN FHUD COMPLEXED WITH GALLICHROME

1EFD の概要

• エントリーDOI: 10.2210/pdb1efd/pdb
• 分子名称: FERRICHROME-BINDING PERIPLASMIC PROTEIN, GALLICHROME (3 entities in total)
• 機能のキーワード: periplasmic binding protein-siderophore complex, fhud complex with gallichrome, escherichia coli, ferric siderophore binding protein, metal transport
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30464.60

構造登録者

Clarke, T.E.,Ku, S.-Y.,Dougan, D.R.,Vogel, H.J.,Tari, L.W. (登録日: 2000-02-07, 公開日: 2000-04-05, 最終更新日: 2024-02-07)

主引用文献

Clarke, T.E.,Ku, S.Y.,Dougan, D.R.,Vogel, H.J.,Tari, L.W.
The structure of the ferric siderophore binding protein FhuD complexed with gallichrome.
Nat.Struct.Biol., 7:287-291, 2000

PubMed Abstract: Siderophore binding proteins play a key role in the uptake of iron in many gram-positive and gram-negative bacteria. FhuD is a soluble periplasmic binding protein that transports ferrichrome and other hydroxamate siderophores. The crystal structure of FhuD from Escherichia coli in complex with the ferrichrome homolog gallichrome has been determined at 1.9 ¿ resolution, the first structure of a periplasmic binding protein involved in the uptake of siderophores. Gallichrome is held in a shallow pocket lined with aromatic groups; Arg and Tyr side chains interact directly with the hydroxamate moieties of the siderophore. FhuD possesses a novel fold, suggesting that its mechanisms of ligand binding and release are different from other structurally characterized periplasmic ligand binding proteins.

PubMed: 10742172
DOI: 10.1038/74048

実験手法

X-RAY DIFFRACTION (1.9 Å)