1EF5

SOLUTION STRUCTURE OF THE RAS-BINDING DOMAIN OF RGL

1EF5 の概要

• エントリーDOI: 10.2210/pdb1ef5/pdb
• 分子名称: RGL (1 entity in total)
• 機能のキーワード: ras-binding domain, rgl, ras, rbd, ra, riken structural genomics/proteomics initiative, rsgi, structural genomics, signaling protein
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11737.15

構造登録者

Kigawa, T.,Endo, M.,Ito, Y.,Shirouzu, M.,Kikuchi, A.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2000-02-07, 公開日: 2000-02-23, 最終更新日: 2024-05-22)

主引用文献

Kigawa, T.,Endo, M.,Ito, Y.,Shirouzu, M.,Kikuchi, A.,Yokoyama, S.
Solution structure of the Ras-binding domain of RGL.
FEBS Lett., 441:413-418, 1998

PubMed Abstract: The RGL protein, a homolog of the Ral GDP dissociation stimulator (RalGDS), has been identified as a downstream effector of Ras. In the present study, the solution structure of the Ras-binding domain of RGL (RGL-RBD) was determined by NMR spectroscopy. The overall fold of RGL-RBD consists of a five-stranded beta-sheet and two alpha-helices, which is the same topology as that of RalGDS-RBD. The backbone chemical shift perturbation of RGL-RBD upon interaction with the GTP analog-bound Ras was also examined. The solution structure of RGL-RBD, especially around some of the Ras-interacting residues, is appreciably different from that of RalGDS-RBD.

PubMed: 9891982
DOI: 10.1016/S0014-5793(98)01596-8

実験手法

SOLUTION NMR