1EF4

SOLUTION STRUCTURE OF THE ESSENTIAL RNA POLYMERASE SUBUNIT RPB10 FROM METHANOBACTERIUM THERMOAUTOTROPHICUM

1EF4 の概要

• エントリーDOI: 10.2210/pdb1ef4/pdb
• 分子名称: DNA-DIRECTED RNA POLYMERASE, ZINC ION (2 entities in total)
• 機能のキーワード: three helix bundle, zinc binding, structural genomics, psi, protein structure initiative, northeast structural genomics consortium, nesg, transferase
• 由来する生物種: Methanothermobacter thermautotrophicus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6509.90

構造登録者

Mackereth, C.D.,Arrowsmith, C.H.,Edwards, A.M.,Mcintosh, L.P.,Northeast Structural Genomics Consortium (NESG) (登録日: 2000-02-07, 公開日: 2000-06-14, 最終更新日: 2024-05-22)

主引用文献

Mackereth, C.D.,Arrowsmith, C.H.,Edwards, A.M.,McIntosh, L.P.
Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum.
Proc.Natl.Acad.Sci.USA, 97:6316-6321, 2000

PubMed Abstract: The RNA polymerase subunit RPB10 displays a high level of conservation across archaea and eukarya and is required for cell viability in yeast. Structure determination of this RNA polymerase subunit from Methanobacterium thermoautotrophicum reveals a topology, which we term a zinc-bundle, consisting of three alpha-helices stabilized by a zinc ion. The metal ion is bound within an atypical CX(2)CX(n)CC sequence motif and serves to bridge an N-terminal loop with helix 3. This represents an example of two adjacent zinc-binding Cys residues within an alpha-helix conformation. Conserved surface features of RPB10 include discrete regions of neutral, acidic, and basic residues, the latter being located around the zinc-binding site. One or more of these regions may contribute to the role of this subunit as a scaffold protein within the polymerase holoenzyme.

PubMed: 10841539
DOI: 10.1073/pnas.97.12.6316

実験手法

SOLUTION NMR