1ECS
THE 1.7 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT ENCODED ON THE TRANSPOSON TN5
Summary for 1ECS
| Entry DOI | 10.2210/pdb1ecs/pdb |
| Related | 1EWJ |
| Descriptor | BLEOMYCIN RESISTANCE PROTEIN, CALCIUM ION, TETRAETHYLENE GLYCOL, ... (4 entities in total) |
| Functional Keywords | arm-exchange, antibiotic inhibitor |
| Biological source | Klebsiella pneumoniae |
| Total number of polymer chains | 2 |
| Total formula weight | 28454.28 |
| Authors | Maruyama, M.,Matoba, Y.,Kumagai, T.,Sugiyama, M. (deposition date: 2000-01-25, release date: 2001-05-02, Last modification date: 2023-08-09) |
| Primary citation | Maruyama, M.,Kumagai, T.,Matoba, Y.,Hayashida, M.,Fujii, T.,Hata, Y.,Sugiyama, M. Crystal structures of the transposon Tn5-carried bleomycin resistance determinant uncomplexed and complexed with bleomycin. J.Biol.Chem., 276:9992-9999, 2001 Cited by PubMed Abstract: The transposon Tn5 carries a gene designated ble that confers resistance to bleomycin (Bm). In this study, we determined the x-ray crystal structures of the ble gene product, designated BLMT, uncomplexed and complexed with Bm at 1.7 and 2.5 A resolution, respectively. The structure of BLMT is a dimer with two Bm-binding pockets composed of two large concavities and two long grooves. This crystal structure of BLMT complexed with Bm gives a precise mode for binding of the antibiotic to BLMT. The conformational change of BLMT generated by binding to Bm occurs at a beta-turn composed of the residues from Gln(97) to Thr(102). Crystallographic analysis of Bm bound to BLMT shows that two thiazolium rings of the bithiazole moiety are in the trans conformation. The axial ligand, which binds a metal ion, seems to be the primary amine in the beta-aminoalanine moiety. This report, which is the first with regard to the x-ray crystal structure of Bm, shows that the bithiazole moiety of Bm is far from the metal-binding domain. That is, Bm complexed with BLMT takes a more extended form than the drug complexed with DNA. PubMed: 11134052DOI: 10.1074/jbc.M009874200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.7 Å) |
Structure validation
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