1EA3

Influenza virus M1 protein

1EA3 の概要

• エントリーDOI: 10.2210/pdb1ea3/pdb
• 関連するPDBエントリー: 1AA7
• 分子名称: MATRIX PROTEIN M1 (2 entities in total)
• 機能のキーワード: influenza virus, matrix protein
• 由来する生物種: INFLUENZA A VIRUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36416.18

構造登録者

Arzt, S. (登録日: 2000-11-03, 公開日: 2001-04-26, 最終更新日: 2023-12-13)

主引用文献

Arzt, S.,Baudin, F.,Barge, A.,Timmins, P.,Burmeister, W.P.,Ruigrok, R.W.
Combined Results from Solution Studies on Intact Influenza Virus M1 Protein and from a New Crystal Form of its N-Terminal Domain Show that M1 is an Elongated Monomeric
Virology, 279:439-, 2001

PubMed Abstract: The amino-terminal domain of influenza A virus matrix protein (residues 1-164) was crystallized at pH 7 into a new crystal form in space group P1. This packing of the protein implies that M1(1-164) was monomeric in solution when it crystallized. Otherwise, the structure of the M1 fragment in the pH 7 crystals was the same as the monomers in crystals formed at pH 4 where crystal packing resulted in dimer formation [B. Sha and M. Luo, 1997, Nature Struct. Biol. 4, 239-244]. Analysis of intact M1 protein, the N-terminal domain, and the remaining C-terminal fragment (residues 165-252) in solution also showed that the N-terminal domain was monomeric with the same dimensions as determined from the crystal structure. Intact M1 protein was also monomeric but with an elongated shape due to the presence of the C-terminal part. Circular dichroism showed that the C-terminal part of M1 contained helical structure. A model for soluble M1 is presented, based on the assumption that the C-terminal domain is spherical, in which the N- and C-terminal domains are connected by a linker sequence which is available for proteolytic attack.

PubMed: 11162800
DOI: 10.1006/VIRO.2000.0727

実験手法

X-RAY DIFFRACTION (2.3 Å)