1EA0

Alpha subunit of A. brasilense glutamate synthase

Summary for 1EA0

• Entry DOI: 10.2210/pdb1ea0/pdb
• Related: 2VDC
• Descriptor: GLUTAMATE SYNTHASE [NADPH] LARGE CHAIN, S-DIOXYMETHIONINE, FLAVIN MONONUCLEOTIDE, ... (5 entities in total)
• Functional Keywords: oxidoreductase, iron sulphur flavoprotein
• Biological source: AZOSPIRILLUM BRASILENSE
• Total number of polymer chains: 2
• Total formula weight: 326786.21

Authors

Binda, C.,Bossi, R.T.,Vanoni, M.A.,Mattevi, A. (deposition date: 2000-11-02, release date: 2001-11-01, Last modification date: 2024-05-08)

Primary citation

Binda, C.,Bossi, R.T.,Wakatsuki, S.,Arzt, S.,Coda, A.,Curti, B.,Vanoni, M.A.,Mattevi, A.
Cross-Talk and Ammonia Channeling between Active Centers in the Unexpected Domain Arrangement of Glutamate Synthase
Structure, 8:1299-, 2000

PubMed Abstract: The complex iron-sulfur flavoprotein glutamate synthase catalyses the reductive synthesis of L-glutamate from 2-oxoglutarate and L-glutamine, a reaction in the plant and bacterial pathway for ammonia assimilation. The enzyme functions through three distinct active centers carrying out L-glutamine hydrolysis, conversion of 2-oxoglutarate into L-glutamate, and electron uptake from an electron donor.

PubMed: 11188694
DOI: 10.1016/S0969-2126(00)00540-2

Experimental method

X-RAY DIFFRACTION (3 Å)