1E8B

Solution structure of 6F11F22F2, a compact three-module fragment of the gelatin-binding domain of human fibronectin

1E8B の概要

• エントリーDOI: 10.2210/pdb1e8b/pdb
• 関連するPDBエントリー: 1E88
• 分子名称: FIBRONECTIN, 2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total)
• 機能のキーワード: extracellular matrix glycoprotein, cell adhesion
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18092.83

構造登録者

Pickford, A.R.,Smith, S.P.,Staunton, D.,Boyd, J.,Campbell, I.D. (登録日: 2000-09-18, 公開日: 2000-10-15, 最終更新日: 2020-07-29)

主引用文献

Pickford, A.R.,Smith, S.P.,Staunton, D.,Boyd, J.,Campbell, I.D.
The Hairpin Structure of the (6)F1(1)F2(2)F2 Fragment from Human Fibronectin Enhances Gelatin Binding
Embo J., 20:1519-, 2001

PubMed Abstract: The solution structure of the (6)F1(1)F2(2)F2 fragment from the gelatin-binding region of fibronectin has been determined (Protein Data Bank entry codes 1e88 and 1e8b). The structure reveals an extensive hydrophobic interface between the non-contiguous (6)F1 and (2)F2 modules. The buried surface area between (6)F1 and (2)F2 ( approximately 870 A(2)) is the largest intermodule interface seen in fibronectin to date. The dissection of (6)F1(1)F2(2)F2 into the (6)F1(1)F2 pair and (2)F2 results in near-complete loss of gelatin-binding activity. The hairpin topology of (6)F1(1)F2(2)F2 may facilitate intramolecular contact between the matrix assembly regions flanking the gelatin-binding domain. This is the first high-resolution study to reveal a compact, globular arrangement of modules in fibronectin. This arrangement is not consistent with the view that fibronectin is simply a linear 'string of beads'.

PubMed: 11285216
DOI: 10.1093/EMBOJ/20.7.1519

実験手法

SOLUTION NMR