1E5U

NMR Representative Structure of Intimin-190 (Int190) from Enteropathogenic E. coli

「1E1B」から置き換えられました 「1INM」から置き換えられました

1E5U の概要

• エントリーDOI: 10.2210/pdb1e5u/pdb
• 分子名称: INTIMIN (1 entity in total)
• 機能のキーワード: intimin, escherichia coli, cell adhesion, outer membrane protein, nmr spectroscopy
• 由来する生物種: ESCHERICHIA COLI
• 細胞内の位置: Cell outer membrane; Single-pass membrane protein: P19809
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20074.33

構造登録者

Prasannan, S.,Matthews, S.J.,Batchelor, M.,Daniell, S.,Reece, S.,Frankel, G.,Dougan, G.,Connerton, I.,Bloomberg, G. (登録日: 2000-08-02, 公開日: 2000-08-16, 最終更新日: 2024-10-16)

主引用文献

Batchelor, M.,Prasannan, S.,Daniell, S.,Reece, S.,Connerton, I.,Bloomberg, G.,Dougan, G.,Frankel, G.,Matthews, S.J.
Structural Basis for Recognition of the Translocated Intimin Receptor (Tir) by Intimin from Enteropathogenic E. Coli
Embo J., 19:2452-, 2000

PubMed Abstract: Intimin is a bacterial adhesion molecule involved in intimate attachment of enteropathogenic and enterohaemorrhagic Escherichia coli to mammalian host cells. Intimin targets the translocated intimin receptor (Tir), which is exported by the bacteria and integrated into the host cell plasma membrane. In this study we localized the Tir-binding region of intimin to the C-terminal 190 amino acids (Int190). We have also determined the region's high-resolution solution structure, which comprises an immunoglobulin domain that is intimately coupled to a novel C-type lectin domain. This fragment, which is necessary and sufficient for Tir interaction, defines a new super domain in intimin that exhibits striking structural similarity to the integrin-binding domain of the Yersinia invasin and C-type lectin families. The extracellular portion of intimin comprises an articulated rod of immunoglobulin domains extending from the bacterium surface, conveying a highly accessible 'adhesive tip' to the target cell. The interpretation of NMR-titration and mutagenesis data has enabled us to identify, for the first time, the binding site for Tir, which is located at the extremity of the Int190 moiety.

PubMed: 10835344
DOI: 10.1093/EMBOJ/19.11.2452

実験手法

SOLUTION NMR