1E5P

Crystal structure of aphrodisin, a sex pheromone from female hamster

1E5P の概要

• エントリーDOI: 10.2210/pdb1e5p/pdb
• 分子名称: APHRODISIN (2 entities in total)
• 機能のキーワード: lipocalin, pheromone, hamster
• 由来する生物種: MESOCRICETUS AURATUS (GOLDEN HAMSTER)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 69556.45

構造登録者

Vincent, F.,Brown, K.,Spinelli, S.,Cambillau, C.,Tegoni, M. (登録日: 2000-07-28, 公開日: 2001-07-26, 最終更新日: 2024-10-16)

主引用文献

Vincent, F.,Lobel, D.,Brown, K.,Spinelli, S.,Grote, P.,Breer, H.,Cambillau, C.,Tegoni, M.
Crystal structure of aphrodisin, a sex pheromone from female hamster.
J.Mol.Biol., 305:459-469, 2001

PubMed Abstract: We have solved the crystal structure of aphrodisin, a pheromonal protein inducing a copulatory behaviour in male hamster, using MAD methods with selenium, at 1.63 A resolution. The monomeric protein belongs to the lipocalin family, and possesses a disulfide bridge in a loop between strands 2 and 3. This disulfide bridge is characteristic of a family of lipocalins mainly identified in rodents, and is analogous to the fifth disulfide bridge of the long neurotoxins, such as alpha cobratoxin. An elongated electron density was found inside the buried cavity, which might represent a serendipitous ligand of unknown origin. The analysis of the water accessible surfaces of the side-chains bordering the cavity indicates that Phe76 may be the door for the natural ligand to access the cavity. This residue defines the entry of the cavity as belonging to the consensus for lipocalins. The face bearing Phe76 might also serve for the interaction with the receptor.

PubMed: 11152604
DOI: 10.1006/jmbi.2000.4241

実験手法

X-RAY DIFFRACTION (1.63 Å)