1E4R

Solution structure of the mouse defensin mBD-8

1E4R の概要

• エントリーDOI: 10.2210/pdb1e4r/pdb
• 分子名称: Beta-defensin 8 (1 entity in total)
• 機能のキーワード: defensin, mouse
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3849.60

構造登録者

Bauer, F.,Schweimer, K.,Kluver, E.,Adermann, K.,Forssmann, W.G.,Roesch, P.,Sticht, H. (登録日: 2000-07-12, 公開日: 2001-07-12, 最終更新日: 2018-06-20)

主引用文献

Bauer, F.,Schweimer, K.,Kluver, E.,Conejo-Garcia, J.R.,Forssmann, W.G.,Rosch, P.,Adermann, K.,Sticht, H.
Structure determination of human and murine beta-defensins reveals structural conservation in the absence of significant sequence similarity.
Protein Sci., 10:2470-2479, 2001

PubMed Abstract: Defensins are cationic and cysteine-rich peptides that play a crucial role in the host defense against microorganisms of many organisms by their capability to permeabilize bacterial membranes. The low sequence similarity among the members of the large mammalian beta-defensin family suggests that their antimicrobial activity is largely independent of their primary structure. To investigate to what extent these defensins share a similar fold, the structures of the two human beta-defensins, hBD-1 and hBD-2, as well as those of two novel murine defensins, termed mBD-7 and mBD-8, were determined by nuclear magnetic resonance spectroscopy. All four defensins investigated share a striking similarity on the level of secondary and tertiary structure including the lack of a distinct hydrophobic core, suggesting that the fold is mainly stabilized by the presence of three disulfide bonds. In addition to the overall shape of the molecules, the ratio of solvent-exposed polar and hydrophobic side chains is also very similar among the four defensins investigated. It is significant that beta-defensins do not exhibit a common pattern of charged and hydrophobic residues on the protein surface and that the beta-defensin-specific fold appears to accommodate a wide range of different amino acids at most sequence positions. In addition to the implications for the mode of biological defensin actions, these findings are of particular interest because beta-defensins have been suggested as lead compounds for the development of novel peptide antibiotics for the therapy of infectious diseases.

PubMed: 11714914
DOI: 10.1110/ps.24401

実験手法

SOLUTION NMR