1E4P

Structure of the ribozyme substrate hairpin of Neurospora VS RNA. A close look at the cleavage site

Summary for 1E4P

• Entry DOI: 10.2210/pdb1e4p/pdb
• Descriptor: RNA (5'-R(*GP*UP*GP*CP*GP*AP*AP*GP*AP*CP*GP*AP*AP* AP*GP*UP*CP*CP*GP*AP*GP*CP*GP*C)-3') (1 entity in total)
• Functional Keywords: rna ribozyme, substrate hairpin
• Biological source: NEUROSPORA SP.
• Total number of polymer chains: 1
• Total formula weight: 7809.76

Authors

Michiels, P.J.A.,Schouten, C.H.J.,Heus, H.A.,Hilbers, C.W. (deposition date: 2000-07-12, release date: 2001-01-16, Last modification date: 2024-05-15)

Primary citation

Michiels, P.J.A.,Schouten, C.H.J.,Hilbers, C.W.,Heus, H.A.
Structure of the Ribozyme Substrate Hairpin of Neurospora Vs RNA: A Close Look at the Cleavage Site
RNA, 6:1821-, 2000

PubMed Abstract: The cleavage site of the Neurospora VS RNA ribozyme is located in a separate hairpin domain containing a hexanucleotide internal loop with an A-C mismatch and two adjacent G-A mismatches. The solution structure of the internal loop and helix la of the ribozyme substrate hairpin has been determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in the internal loop, flanking the cleavage site, a guanine and adenine, are involved in two sheared G.A base pairs similar to the magnesium ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the internal loop and details of the high-resolution structure support the view that the hairpin structure represents a ground state, which has to undergo a conformational change prior to cleavage. Results of chemical modification and mutagenesis data of the Neurospora VS RNA ribozyme can be explained in context with the present three-dimensional structure.

PubMed: 11142381
DOI: 10.1017/S1355838200001394

Experimental method

SOLUTION NMR