1E44

ribonuclease domain of colicin E3 in complex with its immunity protein

Summary for 1E44

• Entry DOI: 10.2210/pdb1e44/pdb
• Descriptor: IMMUNITY PROTEIN, COLICIN E3, 1,2-ETHANEDIOL, ... (4 entities in total)
• Functional Keywords: ribonuclease, inhibition, protein-protein interactions, ribosome inactivation, toxin
• Biological source: ESCHERICHIA COLI; More
• Total number of polymer chains: 2
• Total formula weight: 20784.95

Authors

Carr, S.,Walker, D.,James, R.,Kleanthous, C.,Hemmings, A.M. (deposition date: 2000-06-28, release date: 2001-06-28, Last modification date: 2024-05-08)

Primary citation

Carr, S.,Walker, D.,James, R.,Kleanthous, C.,Hemmings, A.M.
Inhibition of a Ribosome Inactivating Ribonuclease: The Crystal Structure of the Cytotoxic Domain of Colicin E3 in Complex with its Immunity Protein
Structure, 8:949-, 2000

PubMed Abstract: The cytotoxicity of most ribonuclease E colicins towards Escherichia coli arises from their ability to specifically cleave between bases 1493 and 1494 of 16S ribosomal RNA. This activity is carried by the C-terminal domain of the colicin, an activity which if left unneutralised would lead to destruction of the producing cell. To combat this the host E. coli cell produces an inhibitor protein, the immunity protein, which forms a complex with the ribonuclease domain effectively suppressing its activity.

PubMed: 10986462
DOI: 10.1016/S0969-2126(00)00186-6

Experimental method

X-RAY DIFFRACTION (2.4 Å)