1E3M

The crystal structure of E. coli MutS binding to DNA with a G:T mismatch

1E3M の概要

• エントリーDOI: 10.2210/pdb1e3m/pdb
• 分子名称: DNA MISMATCH REPAIR PROTEIN MUTS, 5'-D(*AP*GP*CP*TP*GP*CP*CP*AP*GP*GP*CP*AP*CP*CP*AP* GP*TP*GP*TP*CP*AP*GP*CP*GP*TP*CP*CP*TP*AP*T)-3', 5'-D(*AP*TP*AP*GP*GP*AP*CP*GP*CP*TP*GP*AP*CP*AP*CP* TP*GP*GP*TP*GP*CP*TP*TP*GP*GP*CP*AP*GP*CP*T)-3', ... (6 entities in total)
• 機能のキーワード: dna binding, mismatch recognition
• 由来する生物種: ESCHERICHIA COLI; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 200400.40

構造登録者

Lamers, M.H.,Perrakis, A.,Enzlin, J.H.,Winterwerp, H.H.K.,De Wind, N.,Sixma, T.K. (登録日: 2000-06-19, 公開日: 2000-11-01, 最終更新日: 2024-10-23)

主引用文献

Lamers, M.H.,Perrakis, A.,Enzlin, J.H.,Winterwerp, H.H.K.,De Wind, N.,Sixma, T.K.
The Crystal Structure of DNA Mismatch Repair Protein Muts Binding to a G X T Mismatch
Nature, 407:711-, 2000

PubMed Abstract: DNA mismatch repair ensures genomic integrity on DNA replication. Recognition of a DNA mismatch by a dimeric MutS protein initiates a cascade of reactions and results in repair of the newly synthesized strand; however, details of the molecular mechanism remain controversial. Here we present the crystal structure at 2.2 A of MutS from Escherichia coli bound to a G x T mismatch. The two MutS monomers have different conformations and form a heterodimer at the structural level. Only one monomer recognizes the mismatch specifically and has ADP bound. Mismatch recognition occurs by extensive minor groove interactions causing unusual base pairing and kinking of the DNA. Nonspecific major groove DNA-binding domains from both monomers embrace the DNA in a clamp-like structure. The interleaved nucleotide-binding sites are located far from the DNA. Mutations in human MutS alpha (MSH2/MSH6) that lead to hereditary predisposition for cancer, such as hereditary non-polyposis colorectal cancer, can be mapped to this crystal structure.

PubMed: 11048711
DOI: 10.1038/35037523

実験手法

X-RAY DIFFRACTION (2.2 Å)