1DZ1

Mouse HP1 (M31) C terminal (shadow chromo) domain

1DZ1 の概要

• エントリーDOI: 10.2210/pdb1dz1/pdb
• 関連するPDBエントリー: 1AP0
• 分子名称: MODIFIER 1 PROTEIN (1 entity in total)
• 機能のキーワード: chromatin structure, chromo domain, heterochromatin protein protein interaction, dimeric
• 由来する生物種: MUS MUSCULUS (HOUSE MOUSE)
• 細胞内の位置: Nucleus : P83917
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 16152.36

構造登録者

Brasher, S.V.,Smith, B.O.,Fogh, R.H.,Nietlispach, D.,Thiru, A.,Nielsen, P.R.,Broadhurst, R.W.,Ball, L.J.,Murzina, N.,Laue, E.D. (登録日: 2000-02-11, 公開日: 2000-04-09, 最終更新日: 2024-05-15)

主引用文献

Brasher, S.V.,Smith, B.O.,Fogh, R.H.,Nietlispach, D.,Thiru, A.,Nielsen, P.R.,Broadhurst, R.W.,Ball, L.J.,Murzina, N.,Laue, E.D.
The Structure of Mouse Hp1 Suggests a Unique Mode of Single Peptide Recognition by the Shadow Chromo Domain Dimer
Embo J., 19:1587-, 2000

PubMed Abstract: The heterochromatin protein 1 (HP1) family of proteins is involved in gene silencing via the formation of heterochromatic structures. They are composed of two related domains: an N-terminal chromo domain and a C-terminal shadow chromo domain. Present results suggest that chromo domains may function as protein interaction motifs, bringing together different proteins in multi-protein complexes and locating them in heterochromatin. We have previously determined the structure of the chromo domain from the mouse HP1beta protein, MOD1. We show here that, in contrast to the chromo domain, the shadow chromo domain is a homodimer. The intact HP1beta protein is also dimeric, where the interaction is mediated by the shadow chromo domain, with the chromo domains moving independently of each other at the end of flexible linkers. Mapping studies, with fragments of the CAF1 and TIF1beta proteins, show that an intact, dimeric, shadow chromo domain structure is required for complex formation.

PubMed: 10747027
DOI: 10.1093/EMBOJ/19.7.1587

実験手法

SOLUTION NMR