1DXJ

Structure of the chitinase from jack bean

Summary for 1DXJ

• Entry DOI: 10.2210/pdb1dxj/pdb
• Related: 1CNS 1CTN 1D2K
• Descriptor: CLASS II CHITINASE, SULFATE ION (3 entities in total)
• Functional Keywords: hydrolase, family 19 glycosidase, alpha helical protein
• Biological source: CANAVALIA ENSIFORMIS (JACK BEAN)
• Total number of polymer chains: 1
• Total formula weight: 26235.08

Authors

Hahn, M.,Hennig, M.,Schlesier, B.,Hohne, W. (deposition date: 2000-01-10, release date: 2000-08-29, Last modification date: 2024-10-16)

Primary citation

Hahn, M.,Hennig, M.,Schlesier, B.,Hohne, W.
Structure of Jack Bean Chitinase
Acta Crystallogr.,Sect.D, 56:1096-, 2000

PubMed Abstract: The structure of jack bean chitinase was solved at 1.8 A resolution by molecular replacement. It is an alpha-helical protein with three disulfide bridges. The active site is related in structure to animal and viral lysozymes. However, unlike in lysozyme, the architecture of the active site suggests a single-step cleavage. According to this mechanism, Glu68 is the proton donor and Glu90 assists in the reaction by moving towards the substrate and recruiting a water molecule that acts as the nucleophile. In this model, a water molecule was found in contact with Glu90 O(epsilon1) and Thr119 O(gamma) at a distance of 3.0 and 2.8 A, respectively. The model is in accordance with the observed inversion mechanism.

PubMed: 10957628
DOI: 10.1107/S090744490000857X

Experimental method

X-RAY DIFFRACTION (1.8 Å)