1DXG

CRYSTAL STRUCTURE OF DESULFOREDOXIN FROM DESULFOVIBRIO GIGAS AT 1.8 A RESOLUTION

1DXG の概要

• エントリーDOI: 10.2210/pdb1dxg/pdb
• 分子名称: DESULFOREDOXIN, FE (III) ION (3 entities in total)
• 機能のキーワード: non-heme iron protein, rubredoxin type metal center, electron transport
• 由来する生物種: Desulfovibrio gigas
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7726.39

構造登録者

Archer, M.,Huber, R.,Romao, M.J. (登録日: 1997-07-04, 公開日: 1997-11-12, 最終更新日: 2024-02-07)

主引用文献

Archer, M.,Huber, R.,Tavares, P.,Moura, I.,Moura, J.J.,Carrondo, M.A.,Sieker, L.C.,LeGall, J.,Romao, M.J.
Crystal structure of desulforedoxin from Desulfovibrio gigas determined at 1.8 A resolution: a novel non-heme iron protein structure.
J.Mol.Biol., 251:690-702, 1995

PubMed Abstract: The crystal structure of desulforedoxin from Desulfovibrio gigas, a new homo-dimeric (2 x 36 amino acids) non-heme iron protein, has been solved by the SIRAS method using the indium-substituted protein as the single derivative. The structure was refined to a crystallographic R-factor of 16.9% at 1.8 A resolution. Native desulforedoxin crystals were grown from either PEG 4K or lithium sulfate, with cell constants a = b = 42.18 A, c = 72.22 A (for crystals grown from PEG 4K), and they belong to space group P3(2)21. The indium-substituted protein crystallized isomorphously under the same conditions. The 2-fold symmetric dimer is firmly hydrogen bonded and folds as an incomplete beta-barrel with the two iron centers placed on opposite poles of the molecule. Each iron atom is coordinated to four cysteinyl residues in a distorted tetrahedral arrangement. Both iron atoms are 16 A apart but connected across the 2-fold axis by 14 covalent bonds along the polypeptide chain plus two hydrogen bonds. Desulforedoxin and rubredoxin share some structural features but show significant differences in terms of metal environment and water structure, which account for the known spectroscopic differences between rubredoxin and desulforedoxin.

PubMed: 7666420
DOI: 10.1006/jmbi.1995.0465

実験手法

X-RAY DIFFRACTION (1.8 Å)