1DXE

2-dehydro-3-deoxy-galactarate aldolase from Escherichia coli

1DXE の概要

• エントリーDOI: 10.2210/pdb1dxe/pdb
• 関連するPDBエントリー: 1DXF
• 分子名称: 2-DEHYDRO-3-DEOXY-GALACTARATE ALDOLASE, PHOSPHATE ION, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: class ii aldolase
• 由来する生物種: ESCHERICHIA COLI
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 55250.64

構造登録者

Izard, T.,Blackwell, N.C. (登録日: 2000-01-03, 公開日: 2000-08-25, 最終更新日: 2024-05-08)

主引用文献

Izard, T.,Blackwell, N.C.
Crystal Structures of the Metal-Dependent 2-Dehydro-3-Deoxy-Galactarate Aldolase Suggest a Novel Reaction Mechanism.
Embo J., 19:3849-, 2000

PubMed Abstract: Carbon-carbon bond formation is an essential reaction in organic chemistry and the use of aldolase enzymes for the stereochemical control of such reactions is an attractive alternative to conventional chemical methods. Here we describe the crystal structures of a novel class II enzyme, 2-dehydro-3-deoxy-galactarate (DDG) aldolase from Escherichia coli, in the presence and absence of substrate. The crystal structure was determined by locating only four Se sites to obtain phases for 506 protein residues. The protomer displays a modified (alpha/beta)(8) barrel fold, in which the eighth alpha-helix points away from the beta-barrel instead of packing against it. Analysis of the DDG aldolase crystal structures suggests a novel aldolase mechanism in which a phosphate anion accepts the proton from the methyl group of pyruvate.

PubMed: 10921867
DOI: 10.1093/EMBOJ/19.15.3849

実験手法

X-RAY DIFFRACTION (1.8 Å)