1DU6

SOLUTION STRUCTURE OF THE TRUNCATED PBX HOMEODOMAIN

1DU6 の概要

• エントリーDOI: 10.2210/pdb1du6/pdb
• 分子名称: HOMEOBOX PROTEIN PBX1 (1 entity in total)
• 機能のキーワード: homeodomain, gene regulation
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Nucleus: P41778
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7458.35

構造登録者

Sprules, T.,Green, N.,Featherstone, M.,Gehring, K. (登録日: 2000-01-14, 公開日: 2000-08-16, 最終更新日: 2024-05-22)

主引用文献

Sprules, T.,Green, N.,Featherstone, M.,Gehring, K.
Conformational changes in the PBX homeodomain and C-terminal extension upon binding DNA and HOX-derived YPWM peptides.
Biochemistry, 39:9943-9950, 2000

PubMed Abstract: PBX is a member of the three amino acid loop extension (TALE) class of homeodomains. PBX binds DNA cooperatively with HOX homeodomain proteins that contain a conserved YPWM motif. The amino acids immediately C-terminal to the PBX homeodomain increase the affinity of the homeodomain for its DNA site and HOX proteins. We have determined the structure of the free PBX homeodomain using NMR spectroscopy. Both the PBX homeodomain and the extended PBX homeodomain make identical contacts with a 5'-TGAT-3' DNA site and a YPWM peptide. A fourth alpha-helix, which forms upon binding to DNA, stabilizes the extended PBX structure. Variations in DNA sequence selectivity of heterodimeric PBX-HOX complexes depend on the HOX partner; however, a comparison of five different HOX-derived YPWM peptides showed that each bound to PBX in the same way, differing only in the strength of the association.

PubMed: 10933814
DOI: 10.1021/bi0001067

実験手法

SOLUTION NMR