1DTX

CRYSTAL STRUCTURE OF ALPHA-DENDROTOXIN FROM THE GREEN MAMBA VENOM AND ITS COMPARISON WITH THE STRUCTURE OF BOVINE PANCREATIC TRYPSIN INHIBITOR

1DTX の概要

• エントリーDOI: 10.2210/pdb1dtx/pdb
• 分子名称: ALPHA-DENDROTOXIN, SULFATE ION (3 entities in total)
• 機能のキーワード: presynaptic neurotoxin
• 由来する生物種: Dendroaspis angusticeps (eastern green mamba)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 7549.49

構造登録者

Skarzynski, T. (登録日: 1991-04-29, 公開日: 1992-01-15, 最終更新日: 2024-10-16)

主引用文献

Skarzynski, T.
Crystal structure of alpha-dendrotoxin from the green mamba venom and its comparison with the structure of bovine pancreatic trypsin inhibitor.
J.Mol.Biol., 224:671-683, 1992

PubMed Abstract: The three-dimensional structure of alpha-dendrotoxin (alpha-DTX) from the green mamba (Dendroaspis angusticeps) venom has been determined crystallographically using the method of isomorphous replacement and refined at 2.2 A resolution using a restrained least-squares method. The crystallographic R-factor is 0.169 for all 3451 measured reflections between 7.0 and 2.2 A. Although the main-chain fold of alpha-DTX is similar to that of homologous bovine pancreatic trypsin inhibitor (BPTI), there are significant differences involving segments of the polypeptide chain close to the "antiprotease site" of BPTI. Comparison of the structure of alpha-DTX with the existing models of BPTI and its complexes with trypsin and kallikrein reveals structural differences that explain the inability of alpha-DTX to inhibit trypsin and chymotrypsin.

PubMed: 1373774
DOI: 10.1016/0022-2836(92)90552-U

実験手法

X-RAY DIFFRACTION (2.2 Å)