1DRZ

U1A SPLICEOSOMAL PROTEIN/HEPATITIS DELTA VIRUS GENOMIC RIBOZYME COMPLEX

1DRZ の概要

• エントリーDOI: 10.2210/pdb1drz/pdb
• 分子名称: RNA (HEPATITIS DELTA VIRUS GENOMIC RIBOZYME), PROTEIN (U1 SMALL RIBONUCLEOPROTEIN A), MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: catalytic rna, ribozyme, rna-binding protein, u1a, hdv, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Hepatitis delta virus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34841.53

構造登録者

Ferre-D'Amare, A.R.,Zhou, K.,Doudna, J.A. (登録日: 1998-09-01, 公開日: 1999-02-16, 最終更新日: 2024-10-09)

主引用文献

Ferre-D'Amare, A.R.,Zhou, K.,Doudna, J.A.
Crystal structure of a hepatitis delta virus ribozyme.
Nature, 395:567-574, 1998

PubMed Abstract: The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only catalytic RNA known to be required for the viability of a human pathogen. We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the RNA to bind a small, basic protein without affecting ribozyme activity. The co-crystal structure shows that the compact catalytic core comprises five helical segments connected as an intricate nested double pseudoknot. The 5'-hydroxyl leaving group resulting from the self-scission reaction is buried deep within an active-site cleft produced by juxtaposition of the helices and five strand-crossovers, and is surrounded by biochemically important backbone and base functional groups in a manner reminiscent of protein enzymes.

PubMed: 9783582
DOI: 10.1038/26912

実験手法

X-RAY DIFFRACTION (2.3 Å)