1DQ2

Unlocked metal-free concanavalin A

1DQ2 の概要

• エントリーDOI: 10.2210/pdb1dq2/pdb
• 関連するPDBエントリー: 1DQ0 1DQ1 1DQ4 1DQ5 1DQ6
• 分子名称: Concanavalin-Br, ACETIC ACID (3 entities in total)
• 機能のキーワード: demetallized, lectin, metal-free, unlocked, concanavalin a, sugar binding protein
• 由来する生物種: Canavalia ensiformis (jack bean)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51364.87

構造登録者

Bouckaert, J.,Dewallef, Y.,Poortmans, F.,Wyns, L.,Loris, R. (登録日: 1999-12-29, 公開日: 2000-01-19, 最終更新日: 2024-02-07)

主引用文献

Bouckaert, J.,Dewallef, Y.,Poortmans, F.,Wyns, L.,Loris, R.
The structural features of concanavalin A governing non-proline peptide isomerization
J.Biol.Chem., 275:19778-19787, 2000

PubMed Abstract: The reversible binding of manganese and calcium to concanavalin A determines the carbohydrate binding of the lectin by inducing large conformational changes. These changes are governed by the isomerization of a non-proline peptide bond, Ala-207-Asp-208, positioned in a beta-strand in between the calcium binding site S2 and the carbohydrate specificity-determining loop. The replacement of calcium by manganese allowed us to investigate the structures of the carbohydrate binding, locked state and the inactive, unlocked state of concanavalin A, both with and without metal ions bound. Crystals of unlocked metal-free concanavalin A convert to the locked form with the binding of two Mn(2+) ions. Removal of these ions from the crystals traps metal-free concanavalin A in its locked state, a minority species in solution. The ligation of a metal ion in S2 to unlocked concanavalin A causes bending of the beta-strand foregoing the S2 ligand residues Asp-10 and Tyr-12. This bending disrupts conventional beta-sheet hydrogen bonding and forces the Thr-11 side chain against the Ala-207-Asp-208 peptide bond. The steric strain exerted by Thr-11 is presumed to drive the trans-to-cis isomerization. Upon isomerization, Asp-208 flips into its carbohydrate binding position, and the conformation of the carbohydrate specificity determining loop changes dramatically.

PubMed: 10748006
DOI: 10.1074/jbc.M001251200

実験手法

X-RAY DIFFRACTION (2.05 Å)