1DPI

STRUCTURE OF LARGE FRAGMENT OF ESCHERICHIA COLI DNA POLYMERASE I COMPLEXED WITH D/TMP

1DPI の概要

• エントリーDOI: 10.2210/pdb1dpi/pdb
• 分子名称: DNA POLYMERASE I KLENOW FRAGMENT, ZINC ION (2 entities in total)
• 機能のキーワード: nucleotidyltransferase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 68227.10

構造登録者

Beese, L.,Ollis, D.,Steitz, T. (登録日: 1987-08-11, 公開日: 1987-10-16, 最終更新日: 2024-02-07)

主引用文献

Ollis, D.L.,Brick, P.,Hamlin, R.,Xuong, N.G.,Steitz, T.A.
Structure of large fragment of Escherichia coli DNA polymerase I complexed with dTMP.
Nature, 313:762-766, 1985

PubMed Abstract: The 3.3-A resolution crystal structure of the large proteolytic fragment of Escherichia coli DNA polymerase I complexed with deoxythymidine monophosphate consists of two domains, the smaller of which binds zinc-deoxythymidine monophosphate. The most striking feature of the larger domain is a deep crevice of the appropriate size and shape for binding double-stranded B-DNA. A flexible subdomain may allow the enzyme to surround completely the DNA substrate, thereby allowing processive nucleotide polymerization without enzyme dissociation.

PubMed: 3883192
DOI: 10.1038/313762a0

実験手法

X-RAY DIFFRACTION (2.8 Å)